Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 20X PBS. 20L, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Research Products International Corp Potassium Phosphate, Dibasic, 10 KG
Potassium Phosphate Dibasic, Anhydrous (dipotassium phosphate) is a buffering agent commonly used in molecular biology, biochemistry, and cell culture. It effectively maintains pH in the neutral to slightly alkaline range (7.0–8.0), making it ideal for applications such as protein purification, enzyme assays, and cell culture media. Its strong buffering capacity ensures experimental consistency. In addition to pH control, it serves as a potassium ion source—supporting cellular processes and metabolism. Its dual role as a pH stabilizer and nutrient makes it valuable in media formulation and biochemical research.
Apexbio Technology LLC Annexin V-HF488/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds to double-stranded DNA emitting blue fluorescence and is generally unable to permeate intact cell membranes However during late apoptosis or cell death DAPI can penetrate compromised membranes enabling nuclear staining Therefore combined staining with Annexin V-HF488 and DAPI facilitates the differentiation of cells in different apoptosis stages as well as dead cells
Teknova 1X PBS Solution with 185mM Sodium Chloride, pH 7.4. 1000mL, Sterile 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
Med Vet International Doxycycline Hyclate Tablets (Oracea), 100mg, 50ct
VET LICENSE NEEDS TO BE PROVIDED IN 3-4 BUSINESS DAYS OR ORDER WILL BE CANCELLED
Doxycycline hyclate tablets is a generic version of Oracea. It belongs to a class of antibiotics known as tetracyclines. It should be stored at room temperature between 59-86 degrees F and away from light and moisture.
Alkali Scientific CellPro™ DPBS, 10X, 1L, 12/cs
Experience superior performance and convenience with Alkali Scientific™ CellPro™ 10X DPBS, Dulbecco’s Phosphate Buffered Saline. This concentrated buffer solution offers ten times the strength of standard DPBS, providing researchers with a cost-effective and time-saving option for buffer preparation. With its precisely balanced formulation and high purity, CellPro™ 10X DPBS ensures consistent results in cell culture, molecular biology, and immunocytochemistry applications. Trust Alkali Scientific™ CellPro™ 10X DPBS for reliable performance and reproducibility in your laboratory workflows.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 500ml
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
Medchemexpress LLC NaCl, KCl, Phosphate | AR/GR grade | 10 POUCHES
MCE PBS Powder (2 L of 1x) consists of NaCl, KCl, and Phosphate, and is widely used in immunoassay, microbiology, and protein experiments. The PBS buffer prepared from this product can be directly used for cell culture and other related experiments after being sterilized by 0.22 μm filtration. Each pouch can be diluted to 2 L of 1x PBS buffer, resulting in a concentration of 137 mM NaCl, 2.7 mM KCl, and 10 mM Phosphate. This product offers a convenient method for making PBS buffer, eliminating the need to weigh and mix individual components.
- Consists of NaCl, KCl, and Phosphate.
- Convenient way to make PBS buffer.
- Eliminates the need to weigh and mix individual components.
- All relevant raw materials are AR/GR grade.
- The pH of the 1x PBS buffer is 7.4 ± 0.2 @ 25°C.
![Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P32200-1000.031.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.
Quality Biological Inc DPBS without Calcium and Magnesium, 500 mL
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate Liters ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Electron Microscopy Sciences D-PBS, Formula II Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660121.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are designed for laboratories that need to prepare larger volumes of PBS quickly and accurately. Each tablet dissolves easily in water to create 200 mL of phosphate-buffered saline, a standard buffer used in many biological and biochemical applications. The 500-tablet bottle is perfect for high-throughput labs that require a consistent and reliable buffer solution. These tablets ensure that the PBS solution maintains the correct pH and osmolarity, which is essential for applications such as cell culture, protein assays, and molecular biology experiments. The tablets are convenient, cost-effective, and reduce the chance of errors in solution preparation.